Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b35bfa55a62ddd8b8ef5663c1b60cbcb |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate |
2008-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1168a16978ca86b58b8aacb887295d4f |
publicationDate |
2008-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2008138835-A1 |
titleOfInvention |
Expression cloning method suitable for selecting library clones producing a polypeptide of interest |
abstract |
The present invention relates to methods for producing a recombinant polypeptide of interest, the method comprising the steps of: a) providing a polynucleotide library encoding one or more polypeptides of interest, wherein the library was prepared in an expression cloning vector comprising at least the following elements: i) a polynucleotide encoding a selectable marker; ii) a fungal replication initiation sequence, preferably an autonomously replicating sequence (ARS); and iii) a polynucleotide comprising in sequential order: a promoter derived from a fungal cell, a cloning-site into which the library is cloned, and a transcription terminator; b) transforming a mutant of a parent filamentous fungal host cell with the library, wherein the frequency of non-homologous recombination in the mutant has been decreased compared to the parent; c) culturing the transformed host cell obtained in (b) under conditions suitable for expression of the polynucleotide library; and d) selecting a transformed host cell which produces the polypeptide of interest. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015134691-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015059133-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015134773-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015134737-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015134729-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3293264-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2683732-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107223157-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107223157-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015082535-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015134776-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016100562-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011009700-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10655148-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104837993-A |
priorityDate |
2007-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |