http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0151646-A2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72cde4b491e295395a53ced294df6c13 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
filingDate | 2001-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a57ded0acbd386e85325b01ff22d400e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98a66ce0103c1b2e8bfe693c0d35da4e |
publicationDate | 2001-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0151646-A2 |
titleOfInvention | Methods for producing a polypeptide using a crippled translational initiator sequence |
abstract | The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide; and (b) isolating the polypeptide from the cultivation medium; wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide in tandem with a second nucleic acid sequence comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker, wherein the copy number of the first nucleic acid sequence has been increased by culturing the cell under conditions that select for multiple copies of the selectable marker. The present invention also relates to such fungal host cells and methods for obtaining such fungal host cells. The present invention further relates to nucleic acid constructs and vectors comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03070956-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008138835-A1 |
priorityDate | 2000-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 733.