| abstract |
The current invention provides a process for determining the TCR or BCR receptor gene rearrangements and allows visualization of the repertoire of V(D)J rearranged CDR3 domains on an oligonucleotide array on a solid carrier. The method visualizes the end-result of the V(D)J recombination process and in particular the delet ion and insertion process that takes place in the NDN hypervariable region, where 1 up to 13 nucleotides are deleted from the V, D and/or J gene segments, by using a labeled annealer oligonucleotide that hybridizes on or in close proximity of the NDN region. The method allows detection of the full repertoire or a selected part of the repertoire, by choosing and combining primers that are specific for certain V, D and/or J gene segments flanking the CDR3 on either side. The method of the invention may be used or applied for the diagnosis of an immune response against a pathogen, allergen or auto-allergen. |