abstract |
The invention relates to a nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally said nucleotide sequence comprises control sequences such as: one translational termination sequence orientated in 5' towards 3' direction selected from the following list of sequences: TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5' towards 3' direction selected from the following list of sequences: gctnccyyc, using ambiguity codes for nucleotides: v (A/C/G); n (A/C/G/T), preferably 5'-GCT TCC TTC-3'. The invention further relates to a consensus translational initiator sequence: 5'-mwChkyCAmv-3', preferably the translational initiator sequence is selected from the list consisting of: 5'-mwChkyCAAA-3', 5'-mwChkyCACA-3', and 5'-mwChkyCAAG-3'. |