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filingDate 2004-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43df6933f1375938aff885b87b4e5649
publicationDate 2005-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2005059547-A2
titleOfInvention Assay
abstract The present invention provides an assay kit for detecting an analyte of interest in a sample. The kit comprises a) a reporter species; b) a labeled species having first and second binding regions, wherein the first binding region is capable of binding to the analyte of interest and the second binding region is capable of binding to the reporter species; c) an immobilized species capable of binding to the first binding region of the labeled species; and d) immobilized capture reagent capable of binding to the reporter species. The arrangement is such that the sample is contacted with the labeled species, is then contacted with the immobilized species and is subsequently contacted with the immobilized capture reagent, the reporter species being added prior to exposure of the sample to the immobilized capture reagent. If no analyte is present in the sample, the labeled species becomes bound to the immobilized species and is therefore unable to bind to the immobilized capture reagent. If analyte is present in the sample, the analyte binds to the labeled species such that the labeled species is unable to bind to the immobilized species but can bind to the immobilized capture reagent via the reporter species, the presence of the analyte thus being determined by the presence of labeled species bound to the immobilized capture reagent via the reporter species.
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