| abstract |
The present invention concerns a method for the enrichment of target cells by binding, wherein cell wall binding domains are used. Specifically, the present invention concerns a method for the specific recognition of target cells by binding wherein the CBDs are covalently bound to a solid phase and wherein said solid phase consists of beads, preferably magnetic latex beads. The invention also relates to the use of said method for detection, diagnosis, immobilisation or enrichment of cells. The invention furthermore relates to a reaction kit for a method as described above, wherein said kit comprises additionally to conventional detection means one or more CBDs. Finally, the present invention also relates to biochips, comprising CBDs, specifically biochips comprising two or more different CBDs in defined locations. The cells of the invention preferably are Listeria monocytogenes cells and the CBD is preferably ply500 or ply118 CBD. |