http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004029292-A1

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filingDate 2003-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7b7382d33a1d04eeeb5a9e98d814e9f
publicationDate 2004-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2004029292-A1
titleOfInvention Method of recovering dna
abstract It is intended to provide a method of recovering DNA whereby a target cDNA fragment can be more surely recovered from a cDNA fragment mixture having various types of cDNA fragments. Namely, a method of recovering DNA which comprises the first PCR step wherein a mixture of cDNA fragments each having a first adaptor sequence on one end and a second adaptor sequence on the other end is subjected to PCR by using a first primer having a sequence complementary to the first adaptor sequence together with a labeling and a second primer having a sequence complementary to the second adaptor sequence; the electrophoresis step wherein the cDNA fragments having been amplified in the first PCR step are electrophoresed on a gel; and the recovery step wherein a gel segment containing the target cDNA fragment is cut out from the gel based on the results of the electrophoresis to thereby recover the cDNA fragment.
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