| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ea76778e331e386e0712d02b51c211b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da86d012b0160d4bd183c16697106e6b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d4325462e3222af43d09c7f4b2ef9e53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0808044b6043de0330f3046401e16007 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2011-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2013-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02ba71ac08d1aa5e35f0aad5bfe31099
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98e4fe3bb0e41a454e08a088d3f2a2f4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c321a0a8fdc1d798910e237a8df12f7 |
| publicationDate |
2013-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8404443-B2 |
| titleOfInvention |
Hot start nucleic acid amplification |
| abstract |
Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature. |
| priorityDate |
2004-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |