| abstract |
A method for the production of an enzyme of interest, on an industrial scale, comprising a) fermentation of a microbial strain producing an enzyme of interest in a fermentation medium comprising one or more partially prehydrolysed complex N-source(s), wherein said partially prehydrolysed N-source(s) are sterilised separately from any other source containing carbohydrates, the prehydrolysis being achieved by addition of an acid and/or a hydrolytic enzyme; and b) recovery of the enzyme of interest from the fermentation broth. |