| abstract |
The invention relates to a method for conducting the quantitatively analytical determination of the content of aggregates of substances in mixtures involving the following steps: adding at least one probe provided in the form of a protein, protein fragment, protein analog, peptide, peptide fragment and/or peptide analog, which is capable of binding to the aggregate, to the mixture to be examined, whereby the at least one probe and/or the at least one aggregate have/has at least one detectable property; associating the at least one probe on at least one aggregate before a self-aggregation of the at least on probe prevails, whereby the at least one detectable property undergoes a change during the association of the at least one probe and the at least one aggregate, and; determining the at least one detectable property and/or the modification thereof by using physical methods, whereby, during this determination, the measurement signal obtained is immediately reduced to the level of the background signal, which is caused by non-associated probes and/or aggregates both of which having a detectable property. |