abstract |
The invention relates to a method for analyzing nucleic acid sequences and gene expression. The method is based on the detection of fluorescence signals of nucleotide molecules inserted into growing nucleic acid chains by means of a polymerase. The reaction occurs on a plane surface, wherein a plurality of individual nucleic acid molecules are immobilized on said surface. All said nucleic acid molecules are exposed to the same conditions so that a build-up reaction can simultaneously take place in all nucleic acid molecules. |