| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca1d6d68b8b75bbd7786ae7cd89babc9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_02f4a13a2c72636ce1cf75febff2891f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3475b649dc9ac35104375e846c5d02d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b3ed78d284eb5038600a173f3d21589f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e682720410f6e692101f7f27e3b9059c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P43-00 |
| filingDate |
2001-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_210eb05e77ff47b77ef68a32bebc89d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2dcc3a24ee58dcce8480dea1ce6bf62f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c5819c9202d826363f8b4398e133fd5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_645db881cf54f5b28d33f51637dcd602 |
| publicationDate |
2002-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0248351-A2 |
| titleOfInvention |
A method for in vitro molecular evolution of protein function |
| abstract |
The invention provides a method for generating a polmucleotide sequence or population of sequences fromparent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of a) providing single stranded DNA constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide sequences with an exonuclease to generate populations of single stranded fragments; c) contacting said fragments generated from the plus strands with fragments generated from the minus strands and optionally, adding primer sequences that anneal to the 3' and 5' ends of at least one of the parent polynucleotides under annealing conditions; d) amplifying the fragments that anneal to each other to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics as compared to theone or more protein motifs encoded by said parent polynucleotides. Preferably, the exonuclease is BAL 31. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11149090-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03097834-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8685640-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9676862-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02061142-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02061142-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03097834-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8303945-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8552149-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8173774-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004016811-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004016811-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10640567-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2432366-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011061487-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2432366-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014207064-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9163072-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013034904-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9834589-B2 |
| priorityDate |
2000-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |