Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_494f62265e46bbc2d62dcd13f08a5e1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9a652d3fb63e6921579775d6eb0a3fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad97948cdd048d5564afe99e914a2734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb6fd58f37b088c316f639a096e17c3e |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2002-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d709cc013236c287b5af272905328dfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c1f917160079c6158c8b60e710f3193 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11a383a4d1be060ee1400a371d5d3dbc |
publicationDate |
2002-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-02061142-A2 |
titleOfInvention |
Methods for homology-driven reassembly of nucleic acid sequences |
abstract |
The invention provides methods of forcing recombination between polynucleotides. The methods can include the steps of, (a) generating a single strand of a first polynucleotide; (b) generating a single strand of a second polynucleotide, wherein the second polynucleotide is partially complementary to the first polynucleotide; (c) fragmenting the single strand of the first polynucleotide to generate single stranded first polynucleotide fragments; (d) fragmenting the single strand of the second polynucleotide to generate single stranded second polynucleotide fragments; (e) annealing the single stranded first polynucleotide fragments with the single stranded second polynucleotide fragments; and (f) extending the annealed polynucleotide fragments. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102428182-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004016811-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004016811-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010112840-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102428182-A |
priorityDate |
2001-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |