| abstract |
A method is described for the reversible linking of a natural receptor to a surface, which receptor is present in cell membrane fragments, where the receptor is joined to a coupling substance such as biotin, which can enter into a specific and reversible bond with an antibody, such as anti-biotin, and the antibody is joined to the sensor surface. Specific binding between the receptor and a ligand can be determined by bringing the sensor coated with the receptor into contact with the ligand and measuring the interation between the receptor and the ligand, for example with Mach-Zehnder interferometry. |