| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14c9ad767fb6d5e9dabfb770d4e82252 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-0427
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-0256
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-0422 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54373
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-552 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-552 |
| filingDate |
1990-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_575cee9a7defca8b967074b319f4d405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6dd84bdfbcb18a9392ab3787a8e7217 |
| publicationDate |
1991-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0416730-A2 |
| titleOfInvention |
Substrate preparation for chemical-species-specific binding |
| abstract |
A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0596421-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5527711-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015111765-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2384435-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9744664-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6723516-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9720216-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2384435-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10161934-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8753872-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9216838-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2255637-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0578148-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0578148-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/NL-1014816-C2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0179848-A1 |
| priorityDate |
1989-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |