Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_970070ef362553ceeb63f3e0b0e22491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47d6dec86dd9ddfa566eb69ea70a372e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27c547cf69a02f98dd1f775ad5733bdd |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate |
2000-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9415a8f442feadc379c348aed2dbf2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9bd9ee00540c57a950868a7a443b4d7 |
publicationDate |
2001-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0179546-A2 |
titleOfInvention |
Flavivirus detection and quantification assay |
abstract |
Fluorescent DNA probes specific and flanking primer pairs are designed based on the sequence information found in the conserved terminal 3'-noncoding region of flavivirus, e.g. nucleotides 10653-10678 of dengue virus. Fluorogenic polymerase chain reaction employing these primers and probes produce results that permit specific flavivirus identification. The assays can be both quantitative and qualitative. Optimal assay conditions with zero background are disclosed which permit the detection of low levels of flavivirus from clinical specimens. Specifically, Dengue virus isolates from different geographic regions can be universally detected and identified by the disclosed fluorogenic RT-PCR assay. The fluorogenic RT-PCR assay readily detected viremia in sera collected from individuals ill with dengue fever. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1583949-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1583949-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104357582-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10781495-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9580762-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2366803-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7732169-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3241915-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107893128-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013080307-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7927840-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110846441-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110846441-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2977470-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8759003-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2008200221-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006502747-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011015687-A |
priorityDate |
2000-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |