abstract |
A WNV screening assay is particularly adapted to the needs of a laboratory conducting clinical trials. A first aspect is a hybridization assay probe for nucleic acid detection, comprising a probe sequence having a target complementary sequence of bases, wherein one or more base sequences not complementary to the nucleic acid to be detected are present. Optionally included. A target-complementary sequence of bases consists of 12-87 contiguous bases contained within a specific sequence or its complement, the presence of RNA equivalents, DNA equivalents, nucleotide analogs, and up to 10%, or In addition, taking into account base differences of up to 20%, hybridization assay probes can have a length of up to 100 bases. [Selection] Figure 3 |