Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_087bed7abab91ae0f927879f9fe8b273 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9fd8c08a534b4e030b0252869838e78f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3acf8670babc0ba801847640e53121c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_73a3a3678f4f2b320f5db42a4875c18c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d9df8497b94e574c8e2eb522003dff2 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
filingDate |
2001-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_298c10bcfa0c3a374154ef0cbabc7b07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2dc11a809e11f23a46348edd5125fdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ce628a23c5b12599111e56c0b03fb3f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e2b9322b463003df4adc70360ab1505 |
publicationDate |
2001-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0177368-A1 |
titleOfInvention |
Screening method for the discovery and directed evolution of oxygenase enzymes |
abstract |
The present invention relates to screening methods for oxidation enzymes, particularly mono- and dioxygenases. According to the methods of the invention, a product of an oxidation reaction is converted into a phenol or a catechol, which is easily detected by a Gibbs assay. This conversion allows for a sensitive and efficient assay. Both high-throughput liquid-phase and solid-phase methods using these principles are provided. Also described are methods for detecting phenolic ether-products and sulfhydryl products from oxidation reactions, also using a Gibbs assay. Figure (1) shows this athway for a generic, monosubstituted benzene being degraded by the toluene dioxygenase system. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012028709-A2 |
priorityDate |
2000-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |