| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b6c26dafb838e765db82f0a86d4205e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_782b50542f5bee50339aea69980ec46b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47f0983576e7fb1809697b6a40934d46 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate |
2000-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bc4033008b0cc6c0ed63223a67bc992
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_520f3533369a937a43687d040013ae3e |
| publicationDate |
2000-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0071730-A1 |
| titleOfInvention |
Method for inserting a nucleic acid fragment in a vector, uses thereof and kits therefor |
| abstract |
The invention concerns a method for inserting a nucleic acid fragment in a vector, consisting in: (a) preparing a double-stranded nucleic acid molecule comprising the nucleic acid fragment to be inserted in a vector and on either side of said fragment a nucleic acid label; (b) performing the denaturation of said nucleic acid molecule and of a cleaved vector whereof the 5' and 3' ends of one of the strands are complementary to said nucleic acid labels; (c) incubating said nucleic acid molecule with the cleaved vector in conditions enabling said nucleic acid labels to be hybridised on the cleaved vector; (d) copying the cleaved vector and the nucleic acid fragment; (e) ligating the newly synthesised strands with the free ends; (f) optionally repeating steps (b) through (e). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7056740-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7833759-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7582423-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7235386-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7273739-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7217514-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005010212-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7078211-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7838219-B2 |
| priorityDate |
1999-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |