| abstract |
The invention provides a method for making a glycosyl hydrolase characterized by an increase in catalytic activity on an insoluble substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly binding a disaccharide product in the active site, and a method for making a glycosyl hydrolase characterized by an increase in catalytic activity on a soluble substrate, comprising replacing a hydrophobic substrate binding amino acid of the hydrolase with a positively charged amino acid. The invention further provides a glycosyl hydrolase, comprising Y42R (SEQ. ID NO:1), W82R (SEQ. ID NO:2), or Y245G (1) (SEQ. ID NO:3) and the DNA sequences encoding the enzymes. |