http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9885708-B2

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
filingDate 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a714803f83a7115f4670e683c2ef8009
publicationDate 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9885708-B2
titleOfInvention Methods and combinations of signaling markers for assessment of disease states
abstract Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population.
priorityDate 2012-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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