http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9885708-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_922f5b8a1b062f47489a5f1f29e2e7b2 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54366 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5306 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
filingDate | 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a714803f83a7115f4670e683c2ef8009 |
publicationDate | 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-9885708-B2 |
titleOfInvention | Methods and combinations of signaling markers for assessment of disease states |
abstract | Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population. |
priorityDate | 2012-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.