| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_878e609775831e4b26d97a785054cc41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8844c5d6b41b20eeaf5b5044e12a5c7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a793d2aaaa67cba0527eedac0dd1ae7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6755dca546e9d5a5d6b488aac3107a27
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5e5c121269689cbab9b3a4548b15caf3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b19eaa8e535da4e4bceed99917a68153 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-902 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0068 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 |
| filingDate |
2006-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0088ff9aaed9fe2670e02f61c365f143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d8e89dc793463eb5ca6dcc95cb3f860
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e2c6761e1d2059ee39ba0f03dc42954
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36203e9661b9edf16cebf60e87347d46 |
| publicationDate |
2012-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8137964-B2 |
| titleOfInvention |
Method of producing three-dimensional tissue and method of producing extracellular matrix used in the same |
| abstract |
The present invention provides a novel method of producing a three-dimensional tissue by which cell lamination can be carried out easily. According to the method, a three-dimensional tissue in which cell layers are laminated with an extracellular matrix intervening between each pair of the adjacent cell layers is produced by: (A) forming a cell layer on a substrate; (B) bringing the cell layer formed on the substrate into contact with a solution containing a first substance and a solution containing a second substance alternately, thus forming, on the cell layer, an extracellular matrix in which the first substance and the second substance are laminated alternately; and (C) culturing a cell on the extracellular matrix to form a further cell layer. In the present invention, a combination of the first substance and the second substance is (a) a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence or (b) a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged. |
| priorityDate |
2006-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |