| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34feacf0ddf2f3acee2a8c1dc7a6d25b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-502
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5032 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-77
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate |
2001-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2007-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c937e25788e540765bb7cdafba295508
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e8ab595771e34f20f1badb89b3fa3ab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8449faad584746c9876cf7d70d591b2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a1abdf293372c6bae54e69df04dec96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_768d3208cda74e3759b9eb88b3bea2b9 |
| publicationDate |
2007-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-7282347-B2 |
| titleOfInvention |
Method for extracting quantitative information relating to interactions between cellular components |
| abstract |
A method is described to assay for protein interactions in living cells, e.g. by the introduction of two heterologous conjugates into the cell. The method uses the measurement of cellular distribution of a detectable component (e.g. a GFP-labeled˜fluorescent probe) to indicate the presence or absence of an interaction between that component and a second component of interest. The method uses the knowledge that certain components can be stimulated to redistribute within the cell to defined locations. Inducible redistribution systems make it possible to determine if specific interactions occur between components. Inducible systems are described where it is demonstrated that the redistribution stimuli are essentially “null”, in that they affect no other system in the cell during the assay period, other than the component whose redistribution can be induced. Also described is an extraction buffer which is useful in high throughput screening for drugs which affect the intracellular distribution of intracellular components. The extraction buffer comprises a cellular fixation agent and cellular permeabilisation agent. Optimizing the composition of the extraction buffer and its application to various cell types is described. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10962544-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10761085-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8114615-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9193984-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8597899-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11221333-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9766250-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140099322-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013084950-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009170091-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10018631-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9494595-B2 |
| priorityDate |
2000-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |