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filingDate 2004-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2007-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-7192720-B2
titleOfInvention Array-based epitope-captured antibody display
abstract Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.
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