| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_696a3f4ec2be0b770480a7c53ea92855 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-78 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-165 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate |
2001-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2005-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c71322577924e5f58e11faf2e2445fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0db378351e868a7436445ad06297bda8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14ac7cacdb79fea7667789c96e6915da
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2af12eab88aaa209722907aac705e942
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a57c5d710073232696f306293fe77625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5aaf0908a4999e7ad4a2333214084ca9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54861ae0ab1cf6de9cdcbff45c8559be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c935222649add3e0a461a1112eec9f73 |
| publicationDate |
2005-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-6838555-B2 |
| titleOfInvention |
Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same |
| abstract |
Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained. Also, there is disclosed a pop-out technique in which a recombinant protein expression cassette is inserted into a MOX gene site of the mutant and is allowed to pop out therefrom, thereby utilizing the mutant as a host for general use in producing various proteins of interest. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7262287-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005239074-A1 |
| priorityDate |
1999-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |