| abstract |
A method of distinguishing between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by auto-fluorescent molecules, the method comprising:using plane-polarised light to illuminate a sample containing green fluorescent protein and auto-fluorescent molecules;detecting the intensity of fluorescent light that is emitted with a first polarisation from the sample;detecting the intensity of fluorescent light that is emitted with a second polarisation from the sample; andsubtracting a first of said detected intensities from a second of said detected intensities to obtain a difference signal. |