| abstract |
A device, for use in the electrochemical analysis of an analyte in a small volume liquid sample, having a non-conducting substrate ( 1 ); a conductive layer, deposited on the substrate, in two parts ( 2 a, 2 b ), defining a non-conducting gap ( 8 ) therebetween; an analyte-specific reagent ( 5 ) coated on the conductive layer, on one side of the gap; a reference electrode ( 3 ) on the conductive layer, on the other side of the gap; a spacer layer ( 4 ) deposited over the conductive layer; a monofilament mesh ( 6 ) coated with a surfactant or chaotropic agent, the mesh being laid over the reagent, the reference electrode and the spacer layer; and a second non-conductive layer ( 7 ) adhered to the mesh layer, but not coextensive therewith, thereby providing a sample application area ( 9 ) on the mesh. |