| abstract |
A method and apparatus for reducing the crosstalk between two or more dye channels in a multiple frequency laser induced fluorescence scanner in which two laser beams and associated optics are employed for focusing the two laser beams on a plurality of spatially separated spots, and for collecting and measuring the resulting emission from the sample labeled with at least two dyes. Crosstalk of the dye emission is minimized as the separated spots provide for a more nearly diagonal transformation matrix between signal channels and dye channels. |