| abstract |
The invention discloses a dual-laser-induced fluorescence multicolor detector for single-cell electrophoresis chips. The detector consists of a laser excitation module, a fluorescence collection and detection module, a microscopic imaging module, a signal collection and processing It is composed of a chip detection platform, which has the functions of dual laser-induced fluorescence multi-color detection, normal microscopic observation and alignment observation of dual laser focus spot and detection area on the microfluidic chip. The present invention can realize simultaneous detection and quantification of fluorescent substances with "different light absorption and different fluorescence characteristics", especially "different excitation and different emission" fluorescently labeled multi-component active small molecules in single cells separated by microfluidic chip electrophoresis , greatly expanding the space for related research and application of laser-induced fluorescence detection, microfluidic chips, single-cell analysis, and active small molecule detection. |