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publicationDate 2001-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6261781-B1
titleOfInvention Direct detection and mutation analysis of low copy number nucleic acids
abstract A direct detection and quantitation of RNA or DNA contained in a sample is obtained by capillary electrophoresis of the RNA or DNA hybridized to a DNA or RNA probe of complementary sequence stabilized by the combination of a fluorophore terminally conjugated to the probe and a dye intercalating the RNA-DNA or DNA-DNA hybrid so formed. The RNA or DNA is quantified by measuring the total fluorescence emitted by the electrophoresed hybrid upon excitation by a laser generated light beam.
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