http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6114112-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d48f8c03b846acfb609b826f1a9f3e9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 1998-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57d67634c03a329cab5585da50f0c527 |
| publicationDate | 2000-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-6114112-A |
| titleOfInvention | Method of making immunogenic compositions for infectious bursal disease virus |
| abstract | The present invention provides new methods for preparing compositions for immunizing chickens against IBDV. The method comprises: amplifying RNA extracted from a chicken suspected of having IBDV by using a reverse transcriptase-polymerase chain reaction and a 3'end primer, referred to hereinafter as "Primer 1", which binds to a region of the negative strand of the VP2 gene of IBDV, said region extending from about nucleotide 2402 to about nucleotide 2463 on the negative strand, and a 5' end primer, referred to hereinafter as "Primer 2", which binds to a region of the positive strand of the VP2 gene, said region extending from about nucleotide 1424 to about nucleotide 1489 of the positive strand, to provide a PCR product of from about 733 to about 753 base pairs in length; digesting the PCR product with the restriction enzyme BstNI to provide BstNI restriction fragments; assaying the BstNI restriction fragments to determine the IBDV which has infected the chicken; isolating the IBDV from the chicken; treating the isolated IBDV to produce a killed virus or a live attenuated virus; and combining the killed virus or the live attenuated virus with a pharmacological carrier to provide a composition for immunizing chickens against infection from IBDV, particularly from infection with IBDV in the same class as the isolated virus. In a preferred embodiment, the method further comprises digesting an aliquot of the PCR product with the restriction enzyme MboI to provide MboI restriction fragments; and assaying the MboI restriction fragments to further classify the IBDV which has infected the chicken. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006234652-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107475445-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4690742-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1666608-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007015243-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009208528-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107475445-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7476387-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007128692-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004146530-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005214316-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007010015-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005049794-A3 |
| priorityDate | 1998-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.