| abstract |
Methods and compositions for generating chemiluminescence on reaction with a peroxidase enzyme are provided as well as compounds to be used in the methods and compositions. The compounds have a C-C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups. The compositions contain the double bond-containing compound and a peroxide in an aqueous solution. Additional components which can be included in the compositions are peroxidase activity enhancers, nonionic surfactants or cationic surfactants to improve detection sensitivity of the peroxidase. The chemiluminescent methods and compositions are useful in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs. |