abstract |
PCT No. PCT/DK95/00427 Sec. 371 Date Apr. 25, 1997 Sec. 102(e) Date Apr. 25, 1997 PCT Filed Oct. 26, 1995 PCT Pub. No. WO96/13580 PCT Pub. Date May 9, 1996The invention relates, inter alia, to a DNA construct comprising a DNA sequence which encodes an enzyme exhibiting lipolytic activity and which comprises: a) the DNA sequence shown in SEQ ID No. 1, or b) an analogue of the DNA sequence shown in SEQ ID No. 1 which i) is homologous with the DNA sequence shown in SEQ ID No. 1, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 1, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or which is derived from Humicola insolens DSM 1800. Also disclosed, inter alia, are: lipolytic enzymes encoded by such DNA constructs; and enzyme preparations, detergent additives and detergent compositions comprising such lipolytic enzymes. |