| abstract |
A process for the stable integration of a DNA comprising a gene that is functional in a cell of a cereal plant wherein the DNA is integrated into the nuclear genome of a cereal plant, the process comprising: (a) providing a compact embryogenic callus of the cereal plant, (b) wounding the compact embryogenic callus by cutting the callus into pieces or treating the compact embryogenic callus with a cell wall degrading enzyme for a period of time so as not to cause a complete disruption of tissues and transferring the DNA into the nuclear genome of a cell in the compact embryogenic callus by means of electroporation to generate a transformed cell, and (c) regenerating a transformed cereal plant from the transformed cell. |