Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_13fc77ddfd0903a7118117393f763223 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-958 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-836 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-728 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S977-924 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-703 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate |
1995-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1997-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b348d7f095ff0b63a9c65cdf0054f712 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_661d1aa8090889e2f633f673196d5696 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_332187e71010de73318234716c42a91e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69d0ab80529f876dfc79ef6e2c98a586 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6320ef09061c1afa90ed4fad001002d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c77eb9f89c423f4df6a14b0c8c3e4ae9 |
publicationDate |
1997-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-5702901-A |
titleOfInvention |
Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more DNA's using primers having matched melting temperatures |
abstract |
An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 DEG to about 74 DEG C., while the Tm's are within about 5 DEG C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have Tm's which are greater than 50 DEG C. and are within 15 DEG C. of each other. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009203021-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172836-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7785786-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010015613-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007238095-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8039216-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010291583-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8101362-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010184018-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011045458-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8362227-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100440852-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5858673-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6958210-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005112544-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006019242-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002164586-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004043409-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2390352-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6709813-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7667025-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7893241-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6664053-B1 |
priorityDate |
1993-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |