| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14effe3850d2129f863b969dac4f1db8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-775 |
| filingDate |
1994-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1997-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c85a765b079aae44f6dec122339d40f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44fabf9bf491ffe9151fd4bb407b436f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f9852647def119c1f2210bfbf73655c |
| publicationDate |
1997-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5643757-A |
| titleOfInvention |
High yield production of human apolipoprotein A1 in E. coli. |
| abstract |
Apolipoprotein A1 is prepared in a T7 RNA polymerase/promoter bacterial expression system that optimizes yield and facilitates subsequent purification of the protein. Apolipoprotein A1 cDNA is cloned into an Escherichia coli plasmid containing a bacteriophage T7 RNA polymerase promoter such as a pT or pET plasmid; the recombinant plasmid is transferred to an E. coli strain containing inducible bacteriophage T7 RNA polymerase, e.g., a strain having the enzyme under lac control; T7 RNA polymerase is induced in the strain; and apolipoprotein A1 is isolated from the culture. Preferred embodiments tag the apolipoprotein with a sequence such as a stretch of histidines that facilitates purification subsequent to expression. An especially preferred embodiment employs a pET11d-6His plasmid and E. coli strain BL21(DE)pLysS that produces at least 1 mg protein per liter of culture, preferably up to 2 mg per liter, and the protein can be purified to homogeneity by one-step affinity chromatography on a nickel chelating resin. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7273849-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012109162-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003109442-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3466969-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8119590-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8926958-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005148061-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03064660-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017298367-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2767546-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9745362-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10106801-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2343317-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5858724-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6506879-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009136467-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101921793-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012288893-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0238609-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005287636-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007202081-A1 |
| priorityDate |
1994-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |