Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ca2e4410855da087e11bf3eea8933fa |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0024 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-99 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D501-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
filingDate |
1994-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1995-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d331f6923529543f80c2de2731589a01 |
publicationDate |
1995-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-5424203-A |
titleOfInvention |
Method for selectively deactivating catalase while retaining D-amino acid oxidase activity with a solution having a pH of about 11 to 12 |
abstract |
A method for selectively deactivating catalase while retaining D-amino acid oxidase activity is disclosed. The catalase and the oxidase are both present in whole cells or a cell-free extract. The method comprises combining the whole cells or the cell-free extract with a basic solution at a pH between about 11 and about 12 for a time of about 1 hour and 45 minutes to about 2 hours. The catalase activity is eliminated and the oxidase activity is uneffected. This results in the production of a solution which contains the oxidase and which has no catalase activity. The pH of the solution containing the oxidase is then lowered to provide a D-amino acid oxidase capable of enzymatic oxidation of cephalosporin C to glutaryl-7-ACA in high yields. The whole cells and cell-free extract are preferrably from Triginopsis variablilis. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009208543-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9720921-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6080564-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5559006-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100512774-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6465227-B1 |
priorityDate |
1989-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |