| abstract |
PCT No. PCT/GB90/01597 Sec. 371 Date Apr. 1, 1992 Sec. 102(e) Date Apr. 1, 1992 PCT Filed Oct. 16, 1990 PCT Pub. No. WO91/05872 PCT Pub. Date May 2, 1991.An enhanced chemiluminescent assay, in which a dihydrophthalazinedione such as luminol, a peroxidase such as HRP and an oxidant such as H2O2 are co-reacted in the presence of an enhancer such as p-iodophenol, is modified. The enhancer is generated by enzyme-catalysed reaction of a pro-enhancer, e.g. p-iodophenol phosphate is cleaved by alkaline phosphatase, enabling this enzyme to be assayed instead of peroxidase. Alternatively, the enhancer is added, an anti-enhancer such as p-nitrophenol is generated by enzymatic reaction of a pro-anti-enhancer such as p-nitrophenol phosphate and the reduction in luminescent emission is measured. |