http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5162221-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec5b8c0fc1f827c1360a8f5ea5652225 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-882 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-44 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 |
filingDate | 1990-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1992-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75680882ca7a9e1db4b1b19b45d496a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2a6927dc910b42928b4188a22c30d80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cb66570abc3ccc8b2d68d13515736a3 |
publicationDate | 1992-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-5162221-A |
titleOfInvention | Fructose-1,6-bisphosphate aldolase, a process for the preparation thereof and its use |
abstract | A fructose-1,6-bisphosphate aldolase with obtained from Staphylococcus carnosus is disclosed. The aldolase has considerably improved stability as compared to aldolase from rabbit muscle, having an inactivation rate of 0.77%/d at 25 DEG C. as compared with 59.3%/d for rabbit muscle aldolase. The aldolase is obtained by culturing Staphylococcus carnosus cells, mechanically disrupting the cell mass, and then working up the product by fractional ammonium sulfate precipitation, pH fractionation and ion exchange chromatography to provide F-1,6-BP aldolase with a specific activity of 25 U/mg in 21% yield. Particularly high yields are obtained by the aqueous 2-phase extraction and obtaining the enzyme from the upper phase by anion exchange chromatography. The aldolase is suitable for synthesis of carbohydrates and derivatives thereof by enzymatic reaction of aldehydes with DHAP. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103173421-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5360517-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103173421-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5683897-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006166236-A1 |
priorityDate | 1989-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.