| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0597f9c318d2aeca86db94711c516383 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-27
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
1989-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1992-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d18a706d589b9db552e9e14d9adbb6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd9fad3a2be3c1971bc6998ce1a4bfc0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75295b2f53e27090938ea6ad242beb93 |
| publicationDate |
1992-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5158875-A |
| titleOfInvention |
Production of biologically active insulin-like growth factor i from high expression host cell systems |
| abstract |
A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7625996-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5316932-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003054383-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4219708-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5958764-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008015116-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5407810-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5728676-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7462469-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5695958-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5378613-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010121036-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5708134-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5663304-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8014961-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011045510-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5712249-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008119433-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010035817-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9514034-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6143727-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5808006-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5691168-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008025527-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008025528-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011152188-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5723310-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003064393-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011008904-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5843899-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5683980-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5756672-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5451660-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101511390-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5459052-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8476232-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6033875-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009253628-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5552301-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8552158-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010210547-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011183903-A1 |
| priorityDate |
1989-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |