| abstract |
Methods and compositions are provided for producing polypeptide sequences in high yield by employing DNA constructs, wherein the DNA sequence encoding from the polypeptide of interest in preceded by a leader sequence and processing sequence for secreting and processing said polypeptide. In this manner, the mature polypeptide of interest may be isolated from the nutrient medium substantially free of major amounts of other proteins and cellular debris. The yeast strain S. cerevisiae AB103 (pYEGF8) was deposited on Jan. 5 , 1983, at the A.T.C.C. and given accession No. 20658. The plasmid pY alpha EGF23 (pAB114-pCl/1) was deposited at the A.T.C.C. on Aug. 12, 1983, and given Accession No. 40079. |