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filingDate 1980-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1982-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d964ca3ccb232a9eb025ca43c16737e7
publicationDate 1982-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4337309-A
titleOfInvention Method of deterining the concentration of pancreatic and salivary α-amylase in body fluids
abstract A method of determining the concentration of pancreatic alpha -amylase and salivary alpha -amylase in a body fluid containing a mixture of said alpha -amylases. First and second samples of said body fluid are prepared, and the second samples are treated with an effective amount of an inhibitor capable of selectively inhibiting the activity of said alpha -amylases. The amount of total alpha -amylase, i.e. the sum of salivary alpha -amylase and pancreatic alpha -amylase, is then measured for said first and second samples, and the concentration of salivary and pancreatic alpha -amylase present in said first and second samples is determined by comparing the measured total alpha -amylase values with a standard. The inhibitor used has a discrimination factor-expressed as the ratio between the quantity of inhibitor necessary for reducing the activity of one of said two alpha -amylases by 50% and the quantity of inhibitor necessary for reducing the same activity of the other of said alpha -amylases by 50%-of at least 20:1, preferably at least 50:1, and especially at least 80:1.
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