abstract |
The present invention relates to a method for selective assay of α-amylase isozyme using as a substrate a non-reducing-end and reducing-end modified oligosaccharide, specifically, 4-nitrophenyl O-(4,6-O-benzyliden)-α-D-glucopyranosyl-{(1→4)-O-α-D-glucopyranosyl}₅-(1→4)-α-D-glucopyranoside (hereafter referred to as benzylidene-G₇PNP) rapidly and accurately. |