| abstract |
METHOD FOR THE SELECTIVE SEPARATION AND RECOVERY OF POLYPETIDE SUBSTANCES (E.G. BACTERIAL AND FUNGAL EXOENZYMES, HEME PIGMENTS, POLYPEPTIDE HORMONES) FROM A BIOTIC FLUID (E.G. CULTURE BROTH, BLOOD SERUM, URINE, SAP, EXTRACTS OF DISINTERGRATED CELLS). INVOLVES CONTACTING THE BIOTIC FLUID WITH A MACRORETICULAR RESIN TO EFFECT ADSORPTION OF THE POLYPETIDE, WASHING THE RESIN WITH WATER OR SALT SOLUTION, AND THEN ELUTING THE POLYPETIDE WITH AN AQUEOUS SOLUTION OF AN IONIZING SUBSTANCE AND A MISCIBLE LOW MOLECUALR WEIGHT ORGANIC SOLVENT. THE MACRORETICULAR RESINS EMPLOYED MAY BE COPOLYMERS OF DIVINYLBENZENE WITH STYRENE AND ETHYLVINLYBENZENE OR COPOLYMERS OF TRIMETHYLOLPRPANE TRIMETHYLACRYLATE WITH TRIMETHYLOLPROPANE DIMETHACRYLATE. |