| abstract |
This invention provides libraries of expression constructs having different untranslated regions (UTR) from a genome. The expression constructs include transcription regulatory sequences operably linked with a reporter gene and a 5′ UTR, a 3′ UTR or both, wherein the translation of the reporter gene is under the regulatory control of control regions in the UTR sequence. The libraries of this invention are useful for determining the impact of regulatory sequences in the UTRs on translation of open reading frames under a variety of conditions, such as different cellular environments. |