| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f769b6b3406d6c3d799a58d97d443f2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cba65f2bcb47d21ab4cd4d794224a30a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2005-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb5ab10a767a8e10a7abc06e08bf5786
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13e3748c76e24f53dcd3361b2fa09775 |
| publicationDate |
2007-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2007154892-A1 |
| titleOfInvention |
Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids |
| abstract |
A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (T p ) that allows amplification of the parental nucleic acid to thereby enrich the mutant nucleic acid in the nucleic acid mixture. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101815789-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11008606-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9133490-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11130992-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010203532-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9481881-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104946626-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9447460-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8455190-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11174510-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2008282780-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10913977-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284603-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115391-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2183379-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2183379-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11371090-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2314680-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11174511-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9957556-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2314680-A4 |
| priorityDate |
2005-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |