http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2314680-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_430c89d97f6839f5364904e710af9704 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2009-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39935fd3359520c717001e2ba8c20b7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a311efd9ec9ef765e5cdf1da0193a15e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_badbed9b98669d5447876b4576252a99 |
| publicationDate | 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2314680-A1 |
| titleOfInvention | Method for amplification of target nucleic acid sequence, method for detection of mutation by using the method, and reagents for use in the methods |
| abstract | The present invention provides a method for detecting a mutation capable of detecting a mutation with high sensitivity and high reliability in one reaction system. Using primers (Xmt) and (Xwt), a target nucleic acid sequence whose objective base to be detected is a mutant-type is amplified with amplification efficiency higher than a target nucleic acid sequence whose objective base to be detected is a normal-type. The (Xmt) is a primer that is complementary to a region including a mutant-type base in the template nucleic acid and has a base complementary to a mutant-type base at a 3' region, and the (Xwt) is a primer that is complementary to a region including a normal-type base in the template nucleic acid and has a base complementary to a normal-type base at a 3' region. It is preferable that amplification efficiency by the (Xmt) with reference to a mutant-type template nucleic acid is higher than that by the (Xwt) with reference to a normal-type template nucleic acid. Then, a signal value that shows a molten state of a hybridization product between the thus obtained amplification product and the probe is measured, and the presence or absence of the mutation of the objective base site is determined from a change in the signal value accompanying a change in the temperature. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284603-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115391-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2487632-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012146251-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2487632-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2450443-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9528157-B2 |
| priorityDate | 2008-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.