| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a1807d6c95be41785a3acbf1de3dbdfe |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-501
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate |
2016-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b0a6fd2c82e014b88759e70e5831307
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc30b45bdac0fddd59109b7205a0c200
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3002020f54dccd8415be412d52c0363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2ab9e0d48555fb7f60683d520ad83bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06070c66ae14171a9fc319772ee15748
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5733db7af96d846eb418f1e305dd5e51 |
| publicationDate |
2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10337066-B2 |
| titleOfInvention |
Methods for PCR and HLA typing using unpurified samples |
| abstract |
Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample. |
| priorityDate |
2009-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |