Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2000-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a3a06c3206ff0bb94506ab12baf6fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c266b77b2fb7af2b9a8e2089eaa86080 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3f3d745dd8e089adcaf90b329850d85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed77a65ee55652038672658f660d4987 |
publicationDate |
2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2002101899-A |
titleOfInvention |
DNA mutation detection method and DNA mutation detection device |
abstract |
(57) [Problem] To provide a method and an apparatus for detecting DNA mutation without using gel electrophoresis. SOLUTION: A short oligomer 6 having a length of 5 to 8 bases capable of extending a complementary strand and a long oligomer 61 which hybridizes to a target DNA and has no complementary strand extending ability are hybridized in series with a target DNA 63. And a complementary strand extension reaction 6 starting from a short primer using four nucleic acid substrates and a polymerase. 6, and a step of converting pyrophosphoric acid 68 generated as a reaction by-product of the complementary chain elongation reaction into ATP, performing a luminescence reaction using an enzyme and detecting luminescence, and detecting the presence or absence of a specific sequence. . [Effect] Various items required for DNA mutation measurement can be easily known. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7320866-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7297496-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7348150-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7351539-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10337066-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7396648-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7285387-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7309574-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009131176-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013531469-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007020708-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1546353-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1546353-A4 |
priorityDate |
2000-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |