http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2770208-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32725e94a005113e476561f08f21ac4e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 |
| filingDate | 2021-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6c601cbcb9527c69ef29c19e73eda47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa0873bceca31ada9806732b7b4a5933 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e522536ad97c0ad18e3b5776a6ae02af |
| publicationDate | 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2770208-C1 |
| titleOfInvention | Method for obtaining a heterogeneous preparation based on bromelain, immobilized using ion-exchange resins |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology, namely to a method for producing a heterogeneous biocatalyst based on bromelain immobilized on ion-exchange resins, including adsorption immobilization of bromelain in a buffer solution onto an ion-exchange resin matrix, incubation at room temperature with periodic stirring, washing of the precipitate formed with a buffer until there is no protein in the washing waters, characterized in that immobilization is carried out in the ratio 20 ml of bromelain solution at a concentration of 5 mg/ml per 1 g of air-dry carrier; in this case, immobilization is carried out on an ion exchange resin matrix AB-16-GS, and 0.05 M phosphate buffer, pH 11.0, is used as a buffer solution for immobilization, incubation is carried out for 2 hours, washing of the resulting precipitate is carried out with 0.05 M tris-HCl buffer, pH 7.5. n EFFECT: expansion of the range of methods for obtaining a heterogeneous biocatalyst based on bromelain immobilized on ion-exchange resins. n 1 cl, 5 dwg, 1 ex |
| priorityDate | 2021-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.